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Two main principles are at the basis of flavor formation :
 Enzymatic and fermentation processes
 Non-enzymatic browning reactions or Maillard reaction
A flavor is created in several stages. The flavorist has a large
range of available raw materials and this range is constantly increasing.
The flavorist’s task is to determine which mixture of ingredients
goes well together and gives the best grouping of compounds.
An aroma may be composed of 10, 20, 30 or more different chemical
compounds. These compounds are present in different but specific quantities.
Wine contains approximately 400 different compounds and coffee contains 800.
More than 6000 different natural flavor compounds have so far been
identified and there are estimated to be more than 9000. 95% of these compounds
have a role in flavoring.
The equipment most widely used by the flavorist is the
chromatograph. This tool makes it possible to separate the compounds contained
in a natural food product. The first chromatograph was used in 1900 by a Russian
botanist, Ms. TSWETT to separate plant pigments.
Chromatographic analysis is based on the different speed of
migration of organic compounds through an immobile medium called the « stationary
phase ». This migration takes place in a fluid vector called « mobile phase
».
When the interactions between particles and the stationary phase
are stronger than the interactions between the particles and the phase mobile,
the organic compound is hold back into a column.
Chemical compounds may then be identified by comparing their
retention time, which is the time taken for the chemical compound to pass
through the gas chromatography column alone, and the retention time of a
standard. The combustion of each organic component emits a signal at the level
of a detector. This chromatography detector measures approximately the
percentage of each type of chemical particle present.
To carry on a good chromatography analysis, the first step consist
in selecting the most appropriate sample as possible, remembering that volatile
chemical composition of the fresh raw material is significantly different from
the one of a cooked product. This sample can obviously not be directly injected
into gas chromatograph. Here comes the second step which consist in extracting
the flavor volatile from the raw material, the first objective in aroma analysis
being to ensure that the extract is representative of the original product.
How does one select a technique from the myriad of well-isolation
methods that will be best suited to solution of the current problem ?
The analytical flavor chemist is faced daily with the separation and identification
of complex mixtures. These mixtures comprise a wide range of organic chemicals
that possess varying polarities and reactivities, usually occur in trace
concentrations, and are likely included in other complex organic matrices.
Fortunately, most aroma chemicals are volatile, and procedures for their
isolation from foods and flavors have been established that takes advantage of
this volatility. Not so advantageous is the length of time usually required to
obtain an isolate that is representative of the original aroma or flavor of the
sample. The selection of steam distillation, solvent extraction, trapping of the
volatiles on adsorbents, or combinations of these methods with other techniques
might require several hours before the chemist can begin his chromatographic
separation.
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Flavors are perceived into the brain after a series of changes of
the nervous message generated by volatile compounds in sensory cells of the
nasal cavity. These chemicals achieve into the sensory area, either by a direct
way, when the consumer smells the foodstuff at distance, or by a retro nasal way,
when the foodstuffs are already into the mouth.
Describe a flavor or a taste is not an easy task. It is impossible
for everyone to interpret special terms identically, the reason being that each
person has an individual sense of taste. Flavorists have developed their own
language for that reason. It needs months, indeed years, to learn it.
Furthermore, it evolves and changes following ages and cultures. It is
nevertheless imperative to define the required organoleptic profile. For want of
a precise knowledge of the aromatic language, it is convenient to describe with
the most accuracy as possible the searched notes.
The easiest and the most effective way are to base on examples and
similarities.
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